750 Squadron primarily provides training for the Fleet Air Arm's Observers. After undergoing initial officer training at Britannia Royal Naval College Dartmouth, the student Observer joins 750 Squadron for his flying training. During an intensive seven month course, he learns all aspects of airborne navigation and air traffic control, as well as airmanship, radio and radar techniques, and some basic tactical skills. The course involves many hours of classroom instruction which is afterwards consolidated with one-to-one instructional sorties, either in the computer-controlled navigation simulator or in the air. The course culminates in a series of multi-task tactical navigation exercises in which the student observer's newly acquired skills are tested to their limits.

On completion of the course, the student will graduate to Advanced Flying Training (AFT), having been streamed into the anti-submarine (ASW) or airborne early warning (AEW) Sea King pipelines, or the multi-task Lynx pipeline.

750 Squadron conducts flying training in the Jetstream T Mark 2, a radar equipped version of the civilian Series 200 Jetstream. The aircraft is powered by two Turbomeca Astazou 16D turbo-prop engines, giving a maximum speed of 214 knots at sea level and a service ceiling of 25,000 feet. The minimum crew is one pilot and an observer, but there is a third crew seat in the cockpit and two radar/navigation consoles on the right hand side of the cabin from where the students conduct most of their training. Three additional seats are also available, giving the aircraft a normal passenger capacity of six. Combined with the aircraft's four hour endurance and 1000 mile range, this makes it a useful personnel carrier and the Squadron is often tasked in this role.

The Squadron was formed on 24 May 1939 from the Royal Navy Observer School, initially equipped with Blackburn Shark and Hawker Osprey aircraft, later with Fairey Albacores and Barracudas. It moved to Yeovilton, Somerset, in May 1940 and later that year to Trinidad in the West Indies where it served until disbanded in October 1945 after the war ended. 750 Squadron reformed in April 1952, equipped with the Hunting Percival Sea Prince T1. After several moves, both abroad and in the UK, the Squadron finally settled at Royal Naval Air Station Culdrose, Cornwall. It took delivery of its current Jetstream.